ATP-Regulated Ion Channels in the Plasma Membrane of a Characeae Alga, Nitellopsis obtusa

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ATP-Regulated Ion Channels in the Plasma Membrane of a Characeae Alga, Nitellopsis obtusa.

Using the patch-clamp technique, we recorded single-channel currents across the excised patch of the plasma membrane of Nitellopsis. Both K(+) and Na(+) can pass this channel, but currents were not carried by Cl(-). Upon the addition of ATP or AMP to the cytoplasmic side, the frequency of channel opening decreased. This is the first report on an ATP-regulated channel in plant cells.

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The distribution and rates of exchange of the ions sodium, potassium, and chloride in single internodal cells of the ecorticate characean, Nitellopsis obtusa, have been studied. In tracer experiments three kinetic compartments were found, the outermost "free space" of the cell, a compartment we have called "protoplasmic non-free space", and the cell sap. The concentrations in the vacuole were 5...

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To study the mechanism of aluminium toxicity in plant cells, the effects of aluminium on the electrogenesis of plasma membrane were investigated. We analyzed Al3+ induced membrane potential (Em) changes and potential changes during generation of the action potential (AP) in the internodal cells of Nitellopsis obtusa. Al3+ modified Em decrease significantly. Al3+ didn’t attenuate the amplitude o...

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ION TRANSPORT IN NITELLOPSIS OBTUSA BY ENID A. C. MAcROBBIE AI~D

The distribution and rates of exchange of the ions sodium, potassium, and chloride in single internodal cells of the ecorticate characean, Nitdlopsis obtusa, have been studied. In tracer experiments three kinetic compartments were found, the outermost "free space" of the cell, a compartment we have called "protoplasmic non4ree space", and the cell sap. The concentrations in the vacuole were 54 ...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1990

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.93.1.343